Sampling Methods:

 

 

Abalone and Sea Star Monitoring

 Band Transect/Irregular Plot Protocol

The number and size of seastars (primarily ochre seastars Pisaster ochraceus) and black abalone (Haliotis cracherodii) are monitored along band transects or within irregularly-shaped plots, depending on site topography. Seastar transects are typically 2 x 5 m; abalone transects are typically 1 x 10 m. Transects are marked at both ends (and often in the center) by notched bolts. Irregular plots are marked by four "corner" bolts. All black abalone and ochre seastars in transects or irregular plots are counted and measured (abalone: shell length; seastars: from center to tip of longest ray).

 

Timed Searches

Site-wide timed searches have been employed at locations where abalone and seastars exist in too few numbers to monitor within a limited area. One person spends 30 min (or 2 persons 15 min each) searching crevices and pools along the low intertidal zone haphazardly throughout the site for possible occurrences of ochre seastars or black abalone. Numbers encountered and size measurements (at some sites) are recorded.

 

Owl Limpet Counts and Size Frequencies

The number and size distribution of owl limpets (Lottia gigantea) are monitored each spring and fall to follow population dynamics within 5 permanent circular plots per site at most intertidal sites with sufficient scorable individuals (generally > 20 individuals/plot).  Plots were established in areas of high density to obtain as many measurements as possible, preferably > 100 per site.  Plots are marked with a center bolt, notched to indicate the plot number. Limpets are measured within a circle (typically 1 m radius, 3.14 m2 area) around each bolt. A 1m length of line or tape is attached to the bolt and arced around to form the circle.  Owl limpets found within that circle (including those touched by the 1 m mark) are measured with calipers to the nearest millimeter, then marked with a crayon to avoid scoring duplication. Limpets are never removed from the rock.

 

Photoplots

The cover of target species as well as other core and optional species/taxa/substrates is sampled by photographing 5 (at most sites) permanent 50 x 75 cm plots per target species, then scoring point contact occurrences on the photo image. Plots were established in areas of high target species density wherever possible. Photoplots typically are scored from the photographs in the laboratory, supplemented when possible by field plot sketches and notes. For film photographs, each slide is projected onto a grid of one hundred evenly-spaced points (10 x 10). Individual taxa beneath the points are identified and recorded. When scoring digital images, a grid is created on an LCD computer monitor (using Adobe Photoshop) of one hundred evenly-spaced points (10 x 10), and placed on a separate layer. Layering is not scored separately using either procedure, so the total percent cover is constrained to 100%. The top-most layer that is attached to the substrate (i.e., not an obvious epibiont) is always scored regardless of the type of target or core species involved. Limpets, chitons, abalone, and seastars are scored “as is.” For other motile invertebrates, whatever lies beneath the mobile species is scored if possible; otherwise, the point is scored as “unidentified”. Data are entered into a Microsoft Access database for analysis.

 

Transects

The cover of surfgrass (Phyllospadix spp) is sampled by point-intercepts along 10 m long permanent transects. Transects were established in areas of high surfgrass density wherever possible. Most sites have 3 replicate transects. Transects may run end to end or be separated widely, depending on the shape and expanse of the surfgrass bed. Each transect is divided into 100 points distributed at 10 cm intervals. The top-layer target, core, or optional species/taxon/substrate is scored under each point. In addition, surfgrass is separately scored if it occurs beneath another species.  In the latter situation, total transect cover can be greater than 100%.

 

 

 

 

Updated:  12/9/2004

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